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HPLC Theory



HPLC theory



Here you find tutorials concerning HPLC theory for all levels of knowledge.


HPLC novice


Liquid chromatography in its various forms, where HPLC is the most important and dominant, is of major importance in all areas related to chemistry. Therefore, great many people indirectly come into contact with HPLC and need a basic understanding of its principles. Those of you that are newcomer to the field should look at the Novice section. Here you find a simple and pedagogical introduction to chromatography.


HPLC user


Those of you that use HPLC regularly need to know the terminology and concepts that are used in chromatography. Furthermore, you also need to have a basic understanding of how different experimental parameters affect the separation. It is also important to have a good understanding of the equipment. In the "HPLC user" you will find a collection of the best internet sites for you complemented with some pedagogical material made exclusively for this site. 


HPLC method developer


If you are responsible for the HPLC work in a laboratory or work with development of HPLC methods, the section "HPLC developer" is for you. As a "HPLC developer" you need to be thoroughly familiar with basic chromatographic concepts and their physico-chemical interpretation. You also need to know and understand how the separation i affected by changing the experimental parameters. A thorough technical understanding of the equipment is also needed.


Very good tutorial material which is made specially for you is found at


HPLC scientist


Chromatographic research and advanced method development requires a basic understanding of many areas within physical chemistry and chemical engineering. You need to have a good knowledge about e.g.: The thermodynamic and kinetic models and concepts used in chromatography.  The interpretation of the mass balance equation for chromatography and its consequences. The basic fluid dynamics concepts. Intermolecular forces, properties of solutions etc. In the "HPLC scientist" section you will find a test of your knowledge at this level. You may also find it interesting to read more about reversed phase chromatography at the site


I wish you good luck with your studies in chromatography. Enjoy it, it´s interesting!


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