Are you working with advanced HPLC development?

Here you find some basic material in chromatographic theory.

 

Development of advanced HPLC methods requires a thorough understanding of chromatographic theory. This site will give you a start.

 

We recommend you to first go to the animation in the "HPLC developer" section. Look at the three animations and try to understand the reason for their different outcomes. This is a good start for further studies.

 

Secondly, a good understanding of how the basic mass balance equation is derived is well spent time.

 

From a scientific point of view chromatography comprises several disciplines. Important areas that a chromatographer at this level must be acquainted with include:

- Intermolecular forces.

- Theories of solutions

- Thermodynamics.

- Surface chemistry

- Mass transfer

- Hydrodynamics.

 

Furthermore, a thorough understanding of the implications of the mass balance equation for chromatography is needed. It is also important to have a good understanding of the theory for multicomponent chromatography .

 

In conclusion, a person working at this level needs a good background in physical sciences as well as a technical knowledge about the equipment.

 

Comments or questions?

 

Send an e-mail to studyhplc.com